Evaluation of PCR combined with enzyme-linked immunosorbent assay for detection of Mycoplasmapneumoniae infection in children
PCR 法结合酶联免疫吸附试验用于儿童肺炎支原体感染检验价值评价

摘要

目的 肺炎支原体是由于下呼吸道受到感染的一种主要病原体，当患者患有病原支原体疾病后 会造成局部感染的情况，严重情况下会导致患者的器官以及系统损坏，为了尽早可以开展对症治疗，本文 探析对肺炎支原体感染患者中采用 PCR 法结合酶联免疫吸附试验检测的临床应用价值。方法 入组样本选自 2020 年 1 月-2022 年 5 月期间在我院（川北医学院附属医院检验科）进行检查并治疗的 407 例呼吸道感染患 儿，为所有受检者进行不同的检测方法，分别实施 PCR 法和酶联免疫吸附试验进行检测，比对 PCR 法、酶 联免疫吸附试验指标检测结果。结果 PCR 法检测阳性符合率为 86.36%，酶联免疫吸附试验检测阳性符合 率为 88.19%，P>0.05 说明不存在对比意义。PCR 法与 MP-IgM 两种指标对于肺炎支原体检测结果比较有差 异，P<0.05,有统计学意义。PCR 法和酶联免疫吸附试验对于肺炎支原体检测结果一致性比较差。结论 对儿 童肺炎支原体感染患者进行 PCR 法结合酶联免疫吸附试验检测的临床检验价值极高，能够显著提升诊断准 确率，为医生早期诊断及鉴别提供有价值的参考，对控制病情，改善预后有促进作用，在临床诊治上具有 重要意义。

Abstract

Objective Mycoplasma pneumoniae is a major pathogen caused by infection of the lower respiratory tract. When patients suffer from mycoplasma pneumoniae disease, it will cause local infection. In severe cases, it will lead to the damage of the patient's organs and systems. This article explores the clinical application value of PCR combined with enzyme-linked immunosorbent assay in Mycoplasma pneumoniae infection patients.
Methods The samples selected from January 2020 - May 2022 in our hospital (north sichuan medical college affiliated hospital clinical laboratory) for inspection and treatment of 407 cases of children with respiratory tract infection, for all the subjects to different detection methods, implementation of PCR method respectively and enzyme-linked immunosorbent assay for testing, comparing PCR method, enzyme-linked immunosorbent assay index test results.
Results The positive coincidence rate of PCR was 86.36%, and the positive coincidence rate of enzyme-linked immunosorbent assay was 88.19%, P>0.05 indicating that there was no comparative significance. The results of PCR and MP-igm for Mycoplasma pneumoniae detection were statistically significant (P<0.05). The results of PCR and enzyme-linked immunosorbent assay for Mycoplasma pneumoniae detection were in poor agreement.
Conclusion   the children with mycoplasma pneumoniae infection in PCR method combined with enzyme-linked immunosorbent assay to detect high value for clinical inspection, can significantly improve diagnostic accuracy, doctor for early diagnosis and differential provided valuable reference, to control the condition, improve prognosis has promoting effect, it is of great significance in clinical diagnosis and treatment.